LOD / LOQ Calculator
Calculate the limit of detection and limit of quantitation from a calibration curve or replicate blanks, following ICH Q2 (LOD = 3.3σ/S, LOQ = 10σ/S).
Calibration standards
| Concentration (x) | Signal (y) | |
|---|---|---|
Response standard deviation (σ)
ICH Q2 conventions: 3.3 for LOD, 10 for LOQ.
Limit of detection (LOD)
0.2238
3.3 · σ / S — lowest reliably detectable concentration
Limit of quantitation (LOQ)
0.6781
10 · σ / S — lowest reliably quantifiable concentration
Slope (S)
0.1963
σ (response SD)
0.01331
Intercept
0.01429
R²
0.99895
Formula reference
LOD:
LOD = 3.3 · σ / SLOQ:
LOQ = 10 · σ / Sσ = response standard deviation, S = calibration slope.