PCR Master Mix Calculator

Plan PCR, qPCR, or high-fidelity master mixes with excess volume baked in. Mix component concentrations and fixed-volume reagents.

Bench setup

Enter the number of reactions, your excess factor (10% is typical), and the per-reaction final volume. Add components either by concentration (C₁V₁=C₂V₂) or as a fixed volume per reaction. Water fills the remainder.

Assumptions

Stock concentrations are accurate and freshly mixed.
Master mix is pipetted in equal aliquots before template (or template-free) reagents are added.
Verify pipette accuracy for any per-reaction volume below 1 µL.

Presets:

Number of reactions

Excess (%)

Final reaction volume

µL

Components

Component 1

Mode

Stock concentration

Final concentration

Component 2

Mode

Stock concentration

Final concentration

Component 3

Mode

Stock concentration

Final concentration

Component 4

Mode

Stock concentration

Final concentration

Component 5

Mode

Volume per reaction

µL

Component 6

Mode

Volume per reaction

µL

Your master mix table will appear here

V_per_rxn = (C_final / C_stock) × V_rxn

Formula reference

Per component: V_per_rxn = (C_final / C_stock) × V_rxn

Master mix total: V_total = V_per_rxn × (reactions × (1 + excess/100))